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Immunohistochemical analysis of heme oxygenase‐I in rat liver after ischemia
Author(s) -
Ito Koji,
Ikeda Satoru,
Shibata Toshikazu,
Yano Tomohiro,
Horikawa Saburo
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204351
Subject(s) - heme oxygenase , ischemia , immunohistochemistry , messenger rna , heme , portal vein , medicine , vein , endocrinology , biology , pathology , chemistry , biochemistry , enzyme , gene
Total hepatic ischemia was induced by clamping the hepatic artery, portal vein, and bile duct. After 15 min hepatic ischemia, we examined a time course of the changes in the steady‐state levels of heme oxygenase‐1 (HO‐1) mRNA and protein in the livers. The levels of HO‐1 mRNA was transiently induced and peaked at 4 h after the start of reperfusion. In addition, a pattern of the time course of HO‐1 protein levels was similar to that of HO‐1 mRNA levels. Immunohistochemical analysis clearly showed that the localization of HO‐1 protein induced by hepatic ischemia was restricted to the hepatoeytes in the pericentral vein.