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Single‐strand breaks and repair in nuclear DNA in the presence of hydralazine assayed by the nucleoid technique
Author(s) -
Węglarz Ludmiła,
KocevaChyła Aneta,
Drózdz Marian,
Józwiak Zoifia
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204311
Subject(s) - nucleoid , dna supercoil , dna , dna repair , in vitro , microbiology and biotechnology , incubation , nuclear dna , biophysics , biology , chemistry , biochemistry , dna replication , escherichia coli , mitochondrial dna , gene
The nucleoid sedimentation assay was used to study hydralazine‐induced DNA structural changes and repair in the fibroblasts cultured in vitro. The drug induced a dose dependent loss in negative DNA supercoiling due to the physical breakage of the DNA. Relaxation of supercoiled DNA resulted in the nucleoids sedimenting with lower velocities than those of undamaged control cultures. Repair incubation of the cells did not cause the restoration of DNA supercoiling to control level. Unsuccessful repair of DNA damaged by hydralazine may result in maintaining the damaged DNA in the cell which could have immunologic consequences.