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The increased level of PDGF‐A contributes to the increased proliferation induced by mechanical stimulation in osteoblastic cells
Author(s) -
Wang Wei,
Zhuang Hongming,
Levitz Craig L.,
Fan Huijun,
Seldes Richard M.,
Tahernia A. David,
Brighton Carl T.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204121
Subject(s) - stimulation , platelet derived growth factor receptor , platelet derived growth factor , cell growth , osteoblast , growth factor , chemistry , microbiology and biotechnology , endocrinology , biology , biochemistry , in vitro , receptor
Mechanical stimulation can prompt healing of bone fractures. However, it is largely unknown how osteogenesis is promoted by mechanical stimulation. In this study, we found that mechanical strain‐induced proliferation of osteoblastic cells (MC3T3‐E1) accompanied increased levels of platelet‐derived growth factor‐A (PDGF‐A) mRNA, determined by quantitative reverse transcription/polymerase chain reaction. In addition, neomycin and W‐7, which blocked mechanical strain‐induced proliferation of the osteoblast cells, also blocked mechanical stimulation‐induced elevation of PDGF‐A mRNA. Finally, an antibody against PDGF can inhibit physical stimulation‐induced proliferation of MC3T3‐E1 ceils, suggesting that the increased MC3T3‐E1 cells produced by mechanical stimulation at least partially depends on the increased activity of PDGF.