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Iron as a possible mediator of the oxic‐to‐anoxic transition in Paracoccus denitrificans
Author(s) -
Kucera Igor,
Mat'chová Ivana
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204081
Subject(s) - paracoccus denitrificans , anoxic waters , mediator , chemistry , transition (genetics) , biology , microbiology and biotechnology , biochemistry , environmental chemistry , enzyme , gene
Expression of the lacZ gene from the Fnr‐regulated FF‐melR promoter on a plasmid in iron‐deprived Paracoccus denitrificans cells required not only a decreased oxygen tension but also supplementation with iron. The levels of β‐galactosidase and 5‐aminolevulinate synthase showed comparable responses to changes in iron availability. The presence of soluble and particulate enzymes catalyzing the reduction of Fe(III) by NADH suggests a hypothesis in which the redox state of the cytoplasmic NAD‐couple determines the concentration of free Fe(II) and thereby modulates the activity of a common regulator of the Fnr type.