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Effect of 4‐methylumbelliferone on cell‐free synthesis of hyaluronic acid
Author(s) -
Nakamura Toshiya,
Funahashi Masaru,
Takagaki Keiichi,
Munakata Hidekazu,
Endo Masahiko,
Tanaka Kanji,
Saito Yoshiharu
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700204041
Subject(s) - hyaluronic acid , chemistry , biochemistry , cell , hyaluronan synthase , cell membrane , enzyme , biology , anatomy
Human skin fibroblasts were cultured in the presence of 0.5 mM 4‐methylumbelliferone for 12 h, and cell‐free synthesis of hyaluronic acid was performed using membrane‐rich fraction from the cells. The preincubation of the cells with 4‐methylumbelliferone reduced hyaluronic acid synthesis to 15% of that of non‐preincubated cells, although its chain length was not changed. On the other hand, without preincubation of the cells with 4‐methylumbelliferone, hyaluronic acid synthesis was not changed even when 4‐methylumbelliferone was added directly to the reaction mixture. These results suggest that 4‐methylumbelliferone represses the expression of hyaluronic acid synthase on the cell surface.