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Molecular cloning of rat P38 mitogen‐activated protein kinase and it's osmotic regulation in rat kidney
Author(s) -
Yoshida Takumi,
Sone Masayoshi,
Ogawa Tatsuya,
Nihei Hiroshi,
Ozasa Hisashi,
Tsukada Kinji,
Horikawa Saburo
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203821
Subject(s) - p38 mitogen activated protein kinases , protein kinase a , cloning (programming) , microbiology and biotechnology , mitogen activated protein kinase , chemistry , osmotic shock , kinase , biology , biochemistry , gene , computer science , programming language
Rat p38 mitogen‐activated protein (MAP) kinase cDNA was isolated from rat kidney cDNA library using a PCR cloning strategy. The deduced amino acid sequence consists of 360 amino acids and shares 95.3% similarity with human p38 MAP kinase. The message for rat p38 MAP kinase was about 3.4 kilobases and was highly expressed in the kidney. In water‐deprived rat kidneys, the steady‐state levels of p38 MAP kinase mRNA increased about 2.7‐fold as compared with those of control rats. This result suggests that p38 MAP kinase may play an important role in the osmoregulation in the kidney.