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Micromolar calcium prevents isolated rat liver mitochondria form anoxia‐reoxygenation injury
Author(s) -
Schild Lorenz,
Plumeyer Frank,
Reinheckel Thomas,
Augustin Wolfgang
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203791
Subject(s) - calcium , respiration , mitochondrion , mitochondrial permeability transition pore , oxidative phosphorylation , oxygen , chemistry , membrane potential , oxygene , reactive oxygen species , biochemistry , biophysics , biology , anatomy , apoptosis , programmed cell death , organic chemistry
Rat liver mitochondria were exposed to extramitochondrial free calcium between 0 and 5 μM and/or 5 minutes of anoxia followed by 10 minutes of reoxygenation. At concentrations higher than 4 μM, the membrane potential collapsed indicating the permeability transition of the mitochondrial membrane. Anoxia‐reoxygenation shifted this transition to lower calcium concentrations. Anoxia‐reoxygenation alone resulted in the decrease of ADP stimulated respiration down to about 40 % of its initial value. Between 1 and 2 μM, a protective effect in terms of respiration and oxidative protein modification was found. It is concluced that calcium may suppress the formation of reactive oxygen species during anoxia‐reoxygenation before permeability transition occurs.