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Involvement of caspases in apoptosis induced by ultraviolet B irradiation in A431 human epithelioid tumor cells
Author(s) -
Iwasaki Kazumi,
Izawa Masao,
Mihara Motoyuki
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203741
Subject(s) - apoptosis , caspase , dna fragmentation , proteases , uvb induced apoptosis , programmed cell death , microbiology and biotechnology , a431 cells , biology , caspase 3 , chemistry , enzyme , biochemistry , cell cycle , molecular medicine
Six hours after ultraviolet B (UVB) irradiation (11.6 mJ/cm2), the viability of A431 cells decreased, and, at the same time, fragmentation of genomic DNA into nucleosomal units was observed. Z‐Asp‐CH2‐DCB (100 μM), an inhibitor of interleukin‐1 β‐converting enzyme (caspase‐1 ) and caspase‐1‐like proteases, markedly inhibited UVB‐induced cell death and DNA fragmentation. Both YVAD‐CMK, an inhibitor of caspase‐1, and DEVD‐CHO, an inhibitor of caspase‐3, moderately inhibited the UVB‐induced cell death. A combination of YVAD‐CMK and DEVD‐CHO acted additionally in inhibiting cell death. These observations suggest strongly the cooperative involvement of caspases in the apoptosis induced in A431 cells by UVB.