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Identification of protein kinase C isoforms in marine mussels
Author(s) -
Barcia R.,
LopezGarcia J. M.,
Girardini J. E.,
RamosMartinez J. I.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203711
Subject(s) - mytilus , gene isoform , protein kinase c , mussel , cytosol , biochemistry , isozyme , biology , protein kinase a , kinase , histone , enzyme , chemistry , microbiology and biotechnology , ecology , gene
In this paper we have examined the distribution of some isoforms of protein kinase C in different tissues from the sea mussel Mytilus galloprovincialis Lmk. By immunoblot analysis, we have detected the presence of at least three PKC isoforms, all preferably associated with the cellular cytosolic fraction. The Ca2+‐independent form PKC δ was separated from the Ca2+‐dependent forms (PKC α and β) by means of an ionic change chromatography on DE‐52. A comparative study was carried out on the phosphorylatable non‐artificial substrates. The M.B.P. protein proved to be the best substrate, while the worst was HIIIS histone which, however, is frequently used in evaluation assays of the activity.