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Inhibition of glycohydrolase enzymes by aqueous extracts of Chinese medicinal herbs in a microplate format
Author(s) -
Collins R. A.,
Ng T. B.,
Fong W. P.,
Wan C. C.,
Yeung H. W.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203631
Subject(s) - enzyme , glucuronidase , chemistry , herb , biochemistry , traditional medicine , medicinal herbs , medicine
A microplate assay, for use with a variety of glycohydrolase enzymes, was developed to aid the screening of Chinese medicinal herb extracts for the presence of potential anti‐viral and anti‐lymphoma compounds. The microplate assay method described offers greater convenience, speed and reproducibility over existing methods. The enzymes tested were α‐glucosidase, β‐glucosidase and β‐glucuronidase. The assay can be easily adapted for use with other glycohydrolase enzymes. Of the 12 herb extracts examined four did not inhibit any of the enzymes (<50% inhibition), one inhibited α‐glucosidase only (>50% inhibition), six inhibited β‐glucuronidase only, and one inhibited both α‐glucosidase and β‐glucuronidase. None of the extracts were capable of inhibiting β‐glucosidase to any significant extent.