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Diminished response to food deprivation of the rat brown adipose tissue mitochondrial uncoupling system with age
Author(s) -
GarciaPalmer Francisco J.,
Pericas Jordi,
Matamala Juan C.,
Puigserver Pere,
Bonet María L.,
Palou Andreu,
Gianotti Magdalena
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203621
Subject(s) - brown adipose tissue , thermogenesis , starvation , thermogenin , uncoupling protein , endocrinology , adipose tissue , medicine , mitochondrion , biology , cytochrome c oxidase , white adipose tissue , chemistry , microbiology and biotechnology
The aim of the present work is to investigate the effect of starvation on brown adipose tissue thermogenic activity with aging. Interscapular brown adipose tissue from female Wistar rats of different ages was used; half of them were fed and the other half were starved for 24 hours. Mitochondria were isolated and mitochondrial protein content, GDP‐binding, Cytochrome‐c Oxidase activity and uncoupling protein levels were measured. Results show a decrease of all studied parameters, indicating a diminished thermogenic activity with age. The response to starvation is almost the same in all the parameters studied: a general reduction with starvation and a progressive disappearance of this response to starvation with aging. On the whole, these results would indicate a deficient regulation of brown adipose tissue thermogenic activity in old animals, as it happens in other animal models with an alterated thermogenesis.