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Nucleotide sequence of cDNA fragments coding for the FAD‐, glycerophosphate‐ and calcium‐binding domains of human islet mitochondrial glycerophosphate dehydrogenase
Author(s) -
Novials Anna,
Franco Carmen,
Malaisse Willy J.,
Gomis Ramon
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203591
Subject(s) - complementary dna , gene isoform , biology , biochemistry , microbiology and biotechnology , pancreatic islets , nucleic acid sequence , peptide sequence , islet , nucleotide , gene , mitochondrion , insulin , endocrinology
The cDNA fragments coding for the FAD‐, glycerophosphate‐ and calcium‐ binding domains of mitochondrial glycerophosphate dehydrogenase (mGDH were synthetized using RNA extracted from freshly isolated pancreatic islets of a normal subject and two‐non‐insulin‐dependent diabetic patients. Single strand conformation polymorphism analysis of the PCR products yielded the same mobility as control cDNA probes. Likewise, the nucleotide sequence and corresponding amino acid sequence were identical to the normal gene bank sequence. These findings argue against the presence, in pancreatic islets, of an mGDH isoform distinct from that previously characterized in extrapancreatic organs.