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Oxidation of feric‐cytochrome c by N, N′‐bis (2‐hydroperoxy‐2‐methoxyethyl)‐1,4,5,8‐naphthalene tetracarboxylic diimide, NP III ‐ site specific modification of cytochrome c
Author(s) -
Matsugo Seiichi,
Yasui Fumihiko,
Konishi Tetsuya
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203541
Subject(s) - chemistry , cytochrome c , reagent , photochemistry , cytochrome , oxidative phosphorylation , diimide , naphthalene , stereochemistry , perylene , organic chemistry , molecule , biochemistry , enzyme , mitochondrion
When the synthetic hydroxyl radical generator, N, N′‐bis (2‐Hydroperoxy‐2‐methoxyethyl)‐1,4,5,8‐naphthalene‐tetracarboxylic diimide, NP III, was photoirradiated in the presence of ferric‐cytochrome c (Fe3 +), the fragmentation peak corresponding to the specific oxidative modification at the histidine site of cytochrome c was observed in the Matrix‐Assisted Laser Desorption Ionization‐Time of Flight Mass Spectroscopy (MALDI‐TOF) measurement. Without photoirradiation, no such protein oxidation was observed in the experimental conditions employed. This result suggests the possible usage of NP‐III as an oxidative protein‐modifying reagent.