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Oxidized low‐density‐lipoprotein triggers programmed death of endothelial cells
Author(s) -
Seelos Christian,
Gmeiner Bernhard,
Kapiotis Stylianos,
Cerni Christa
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203471
Subject(s) - programmed cell death , fragmentation (computing) , apoptosis , umbilical vein , microbiology and biotechnology , endothelial stem cell , incubation , chemistry , lipoprotein , dna fragmentation , prophase , lactate dehydrogenase , biology , biochemistry , in vitro , enzyme , cholesterol , ecology , meiosis , gene
Incubation of bovine aortic as well as human umbilical vein endothelial cells with either oxidized or native low‐density‐lipoprotein in the presence of trace amounts of copper induced morphological changes of the cells and chromatin fragmentation characteristic for programmed cell death. Shrinkage of cells was evident after 6 to 8 hours of incubation and clearly preceded release of lactate dehydrogenase as a marker of cell permeability. Condensation of nuclear chromatin and internucleosomal cleavage was demonstrated by Hoechst staining and gel electrophoresis, respectively. Thus, by inducing active death of endothelial cells oxidized low‐density‐lipoprotein might negatively influence tissue homeostasis of the endothelium and thereby promote the development of atherosclerotic plaques.