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Study of peptide‐protein interaction by high‐performance liquid chromatography. Effect of hydrophobic and steric parameters
Author(s) -
Szikszay Zoltán,
Forgács Esther
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203091
Subject(s) - steric effects , chemistry , peptide , hydrophilic interaction chromatography , chromatography , hydrophobic effect , high performance liquid chromatography , stereochemistry , organic chemistry , biochemistry
The interaction of 16 peptides was studied on zein‐coated silica column by high‐performance liquid chromatography. The separation factor, theoretical plate number and the asymmetry factors of the peaks were calculated. Stepwise regression analysis was applied to study the peptide‐protein interactions and to find the molecular substructures involved in the interactions and to define their character. Zein‐coated silica column was suitable for the determination of peptide‐protein interaction and the investigated peptide‐protein interactions were found to be mixed (hydrophobic and steric) character.