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Staurosporine‐induced apoptosis of immortalized mouse proximal tubule cells is modulated by bcl‐2 expression level
Author(s) -
Takeda Michio,
Kobayashi Mami,
Osaki Takako,
Shirato Isao,
Endou Hitoshi
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700203061
Subject(s) - staurosporine , apoptosis , genistein , dna fragmentation , microbiology and biotechnology , kinase , protein kinase c , chemistry , protein kinase inhibitor , cell cycle , protein kinase a , biology , programmed cell death , endocrinology , biochemistry
Mouse terminal proximal tubule (S3)cells treated with staurosporine (STS) underwent morphological and biochemical changes characteristic of apoptosis. Treatment of S3 cells with STS, H‐7, PMA, herbimycin A or genistein caused DNA fragmentation. STS inhibited the activity of protein kinase C but not of cdc‐2 kinase in S3 cells. No change in cell cycle distribution was observed in S3 cells treated with STS. However, treatment of S3 cells with STS resulted in a decrease in the level of bcl‐2 mRNA expression in the cells. Overexpression of bcl‐2 inhibited the degree of STS‐induced apoptosis of S3 cells. In conclusion, STS induces apoptosis of mouse S3 cells via inhibition of various protein kinases including protein kinase C, and the apoptosis is modulated by the bcl‐2 expression level.