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Regulation of androgen receptor (AR) and prostate specific antigen (PSA) expression in the androgen‐responsive human prostate LNCaP cells by ethanolic extracts of the Chinese herbal preparation, PC‐SPES
Author(s) -
Hsieh Tzechen,
Chen Sophie S.,
Wang Xuhui,
Wu Joseph M.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202941
Subject(s) - lncap , androgen receptor , prostate cancer , proliferating cell nuclear antigen , cell growth , western blot , androgen , prostate specific antigen , chemistry , antigen , intracellular , prostate , endocrinology , medicine , cancer research , biology , cancer , biochemistry , hormone , immunology , gene
Abstract As part of the study on the potential use of natural product‐based combination therapy for treating prostate cancer, we have investigated the effects of a “HPLC standardized” herbal preparation, PC‐SPES, on the prostate LNCaP cell line. Proliferation of the LNCaP cells was inhibited by a 4‐6 day incubation with ethanolic extracts of PC‐SPES. Decrease of cell growth was accompanied by a 60‐70 % down‐regulation of the proliferating cell nuclear antigen (PCNA) and level of secreted PSA. A smaller and more variable decrease (20‐40%) in the level of intracellular PSA was also observed. The PC‐SPES‐modulated PSA changes occurred concurrently with the decrease of AR expression, based on Western blot analysis and binding to the radioactive ligand [3H]R1881. A 60% decrease in R1881 binding occurred after a 24 h incubation with PC‐SPES. These results suggest that PC‐SPES negatively affects cell growth in part through its ability to modulate changes in PCNA, and may decrease PSA levels indirectly by suppressing AR expression.