Premium
Preincubation of thymocytes with 1‐(5‐isoquinolinesulfonyl)‐2‐methylpiperazine dihydrochloride (H‐7) induces apoptosis in non‐stimulated thymocytes
Author(s) -
Sakurai Yoshiaki,
Hirata Chihiro,
Onishi Yoshiaki,
Tanimoto Yutaka,
Yamada Takeshi,
Kizaki Harutoshi
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202841
Subject(s) - apoptosis , thymocyte , protein kinase c , kinase , gene isoform , microbiology and biotechnology , chemistry , hydrochloride , biology , immunology , biochemistry , t cell , gene , immune system
1‐(5‐Isoquinolinesulfonyl)‐2‐methylpiperazine hydrochloride (H‐7), an inhibitor of protein kinases, has been shown to inhibit the thymocyte apoptosis induced by various apoptogenic agents. In the present study, when mouse thymocytes were pretreated with H‐7, washed, and cultured for an additional time, apoptosis was induced depending on the preincubation time and the dose of H‐7. The protein kinase C activity in the H‐7‐pretreated and ‐washed cells was not altered, suggesting that an alteration of a certain PKC isoform is related to both the triggering and the progression of apoptosis.