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Variable requirement for splicing signals for nucleocytoplasmic export of mRNAs
Author(s) -
Fu Litao,
Suen Christine K. M.,
Waseem Ahmed,
White Kenneth N.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202731
Subject(s) - rna splicing , nuclear export signal , cytoplasm , biology , microbiology and biotechnology , alternative splicing , precursor mrna , nucleus , messenger rna , intron , cell nucleus , splicing factor , hamster , gene expression , gene , rna , genetics
Using in situ hybridisation to detect tile intracellular localisation of mRNAs we have found that mRNAs expressed from intronless cDNAs of normally intronic genes are expressed well but largely retained ill nuclei. The degree of nuclear retention is quite variable but in all cases addition of splicing signals to the expression cassette are required for efficient export of the mRNAs from nucleus to cytoplasm. In contrast mRNAs expressed from the intronless genes of hamster β‐adrenergic receptor and human serotonin receptor type 1A showed very little nuclear accumulation and strong expression in the cytoplasm independently of splicing signals. The data demonstrate a link between splicing and export and dissemble from the idea that splicing enhances mRNA expression by protecting nascent nuclear mRNAs from degradation.