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Expression of soluble, active human macrophage colony stimulating factor in Escherichia coli
Author(s) -
Jiang Xueyuan,
Zhou Aiwu,
Cen Bo,
Qiu Qifeng,
Dong Xueyin,
Xu Xianxiu
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202721
Subject(s) - recombinant dna , escherichia coli , macrophage colony stimulating factor , microbiology and biotechnology , macrophage , amino acid , biology , colony stimulating factor , gene , gene expression , expression vector , biochemistry , chemistry , in vitro , stem cell , haematopoiesis
Human macrophage colony stimulating factor (M‐CSF) has been successfully overexpressed in Escherichia coli AD494 (DE3) with an expression level of approximate 26 % of the total cellular proteins. The truncated human M‐CSF gene encoding the amino‐terminal 149 amino acids was subcloned into the prokaryotic expression vector pET11d under the control of the inducible T7 promoter. Nearly 40 % of the recombinant protein was in the soluble fraction which showed obvious stimulating effects on mouse macrophage colony formation and had an M‐CSF specific activity of ≈1×106 units/mg soluble protein.