Premium
A specific assay for discriminating between peroxidase and lipoxygenase activities
Author(s) -
Fontana Mario,
Costa Mara,
Mosca Luciana,
Rosei Maria Anna
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202541
Subject(s) - peroxidase , lipoxygenase , enzyme , chemistry , heme , biochemistry
Peroxidases are widespread heme‐containing enzymes able to catalyze the oxidation of a large array of organic substrates. There is growing interest in the measurements of peroxidase activity. We noticed that many substrates used in the routine assays for the biological and cytological determinations of peroxidase could be oxidized by lipoxygenase. We found interesting to set up a procedure to detect selectively peroxidase. In the present note, we report a fluorometric test for peroxidase detection using phenolic compounds or hydroxycoumarins.