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cDNA sequence analysis of cardiotoxin variants from Taiwan cobra
Author(s) -
Chang LongSen,
Lin Jordge,
Wu PeiFung
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202461
Subject(s) - cardiotoxin , cobra , naja , venom , complementary dna , biology , gene , peptide sequence , amino acid , ophidia , rna , homology (biology) , microbiology and biotechnology , biochemistry , genetics , computer science , programming language
Five cDNAs encoding cardiotoxin variants were constructed from the cellular RNA isolated from the venom glands of Naja naja atra by reverse transcription‐polymerase chain reaction. A high degree of nucleotide sequence homology was observed between these variants and other determined ones. Among them, a novel cardiotoxin 6 had 61 amino acid residues rather than 60 ones that usually observed with Naja naja atra cardiotoxins. The other cardiotoxin variants were the homologues of cardiotoxins 1, V or N with one or two amino acid substitutions, respectively. These results probably reflect the involvement of RNA editing in the production of cardiotoxin variants in the venom of Taiwan cobra.