Premium
High salt and solvent induced Z‐conformation in native calf thymus DNA
Author(s) -
Ali Naushad,
Ali Rashid
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202321
Subject(s) - salt (chemistry) , dna , solvent , chemistry , crystallography , stereochemistry , biochemistry , organic chemistry
The Z‐DNA forming potentiality of native calf thymus DNA using spectrophotometric measurements and diethyl pyrocarbonate reactivity have been investigated. The [image omitted] value was evaluated from absorbance ratio for B‐ and Z‐DNA and compared with the values obtained for native DNA. The results suggest that native DNA undergoes B‐ to Z‐/Z‐analogous transition to an extent of 20 per cent under constraint conditions. Possible Z‐DNA formation was also characterized by hyper‐reactivity of native DNA towards diethyl pyrocarbonate in aqueous solvent. The thermal melting profile of native and modified DNA and nuclease S1 digestibility data, in addition, reflects B‐ to Z‐/Z‐analogous transition in native DNA.