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Complete sequence of the upp gene encoding uracil phosphoribosyltransferase from Mycobacterium bovis BCG
Author(s) -
Kim Jin Koo,
Kim Jeong Hyun,
Lee SeungHwan,
Kang Young,
Lim JongSeok,
Lee Hee Gu,
Kim Sang Jae,
Bai Gill Han,
Park Young Kil,
Cho SangHyun,
Kang Sang Won,
Kim Yu Sam,
Choe In Seong,
Chung Tai Wha,
Park SueNie,
Kim Seung Ho,
Ahn Jong Seog,
Choe YongKyung
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202201
Subject(s) - mycobacterium bovis , gene , biology , sequence (biology) , genetics , medicine , mycobacterium tuberculosis , pathology , tuberculosis
The nucleotide sequence of a 799 bp fragment of Mycobacterium bovis BCG containing the putative upp genc that encodes a uracil phosphoribosyltransferase (UPRTase [EC 2.4.2.9]) has been determined. The upp gene of BCG has an open reading frame (ORF) of 621 bp (207 amino acids) starting with GTG (position 112) and ending with TGA (position 733), and its molecular mass was calculated to be 21,864 Da. Comparative analyses of the deduced amino acid sequence of BCG UPRTasc with the UPRTase of six bacterial genera revealed that 24% (50/211) of the residues are perfectly conserved and 32% (67/211) of the residues arc well conserved.