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Induction of mRNAs for ICAM‐1, VCAM‐1, and ELAM‐1 in cultured rat cardiac myocytes and myocardium in vivo
Author(s) -
Hattori Yoshiyuki,
Kasai Kikuo
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700202041
Subject(s) - vcam 1 , cell adhesion molecule , myocyte , icam 1 , in vivo , intercellular adhesion molecule 1 , microbiology and biotechnology , biology , lipopolysaccharide , messenger rna , intracellular , adhesion , cell adhesion , gene expression , chemistry , immunology , gene , cell , biochemistry , organic chemistry
We evaluated the expression of mRNAs for intercellular adhesion molecule‐1 (ICAM‐1), vascular cell adhesion molucule‐I (VCAM‐1), and endothelial leukocyte adhesion molecule‐1 (ELAM‐1) in cultured neonatal rat cardiac myocytes and in the rat heart by the reverse transcription‐polymerase chain reaction with gene specific primers. Bacterial lipopolysaccharide (LPS) and interferon‐γ induced gene expression for ICAM‐t, VCAM‐1, and ELAM‐1 in cultured cardiac myocytes. While mRNA for any of these adhesion molecules was very low or negligible in hearts of control rats, the three mRNAs were substantially induced in hearts 3h after the injection of LPS. Results show that mRNAs for the three inducible adhesion molecules are expressed in cultured cardiac myocytes and rat heart tissues and are enhanced by immune stimulation, suggesting their involvement in cardiac inflammatory disorders.