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Effect of cyclins and Cdks on the cyclin B1 promoter activation
Author(s) -
Moro A.,
Zerfass K.,
Joswig S.,
JansenDuerr P.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201971
Subject(s) - cyclin a2 , cyclin d , cyclin dependent kinase , cyclin a , cyclin d1 , cyclin b , cyclin , cyclin e , cyclin dependent kinase complex , microbiology and biotechnology , cancer research , chemistry , biology , cell cycle , biochemistry , gene
To study the effect of several cyclins on cyclin B1 promoter activation, we co‐transfected cyclin A, cyclin E and cyclin D1 expressing plasmids with a cyclin B1 promoter construction driving a luciferase repotnter gene into NIH 3T3 cells. All three cyclins produced activation of the reporter gene, however, co‐transfection of cyclin A with a Cdk2 dominant negative mutant blocks activation by cyclin A. Our results suggest that cyclin B1 activation depends on Cdk2 kinase activity associated to cyclin A. To our knowledge this is the first report showing positive effect of cyclin A, cyclin E and cyclin D1 on cyclin B1 activation and blocking of this activation by a Cdk2 dominant negative mutant.