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Molecular cloning and growth hormone‐regulated gene expression of carp insulin‐like growth factor‐I
Author(s) -
Hashimoto Hisashi,
Mikawa Satoshi,
Takayama Eiji,
Yokoyama Yoshihiro,
Toyohara Haruhiko,
Sakaguchi Morihiko
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201921
Subject(s) - carp , complementary dna , signal peptide , biology , open reading frame , microbiology and biotechnology , amino acid , insulin like growth factor , peptide sequence , northern blot , peptide , cdna library , gene expression , gene , molecular cloning , cloning (programming) , growth factor , biochemistry , receptor , fishery , fish <actinopterygii> , computer science , programming language
Two distinct insulin‐like growth factor‐I (IGF‐1 cDNAs were isolated from a juvenile carp liver cDNA library. Both of the cDNAs encoded a full length prepro IGF‐I with 161 amino acids: a mature peptide (70 residues), its signal peptide (44 residues), and a carboxy‐terminal E domain (47 residues). The similarity of the two cDNA in the open reading frame was 95.1%. The amino acid sequences of E domain predicted by the two cDNAs were different while those of the signal peptide and the mature peptide were the same. By Northern blot analysis, four different sizes (1, 1.5, 2, and 4.5kb) of IGF‐I mRNA were recognized in the liver of juvenile carp. The two smaller species (lkb and 1.5kb) were not detected in adult carp. The expression of these two species was preferably induced by exogenous growth hormone administration in the liver of juvenile carp.