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Promotion of proliferation of murine BALB/c3T3 fibroblasts mediated by nitric oxide at lower concentrations
Author(s) -
Du Ming,
Islam Md. Muzharul,
Lin Ling,
Ohmura Yoshihisa,
Moriyama Yoichi,
Fujimura Shinji
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201661
Subject(s) - snap , nitric oxide , s nitrosoglutathione , chemistry , thymidine , microbiology and biotechnology , cell growth , in vitro , biochemistry , biology , glutathione , enzyme , computer graphics (images) , organic chemistry , computer science
This study showed that nitric oxide (NO)‐generating S‐nitrosothiols, S‐nitroso‐N‐acetylpenicillamine (SNAP) and S‐nitrosoglutathione (GSNO), increased proliferation of BALB/c 3T3 (clone A31‐1‐1) fibroblasts in vitro. Treatment with SNAP at a relatively low concentration (0.005‐0.02 mM) induced an increase in cell number compared to control. SNAP (0.005‐0.02 mM) and GSNO (0.025‐0.05 mM) both showed an increase of [3H]thymidine incorporation in exponentially growing cells in a dose‐dependent manner. The maximal effect was observed at about 40 and 90% above control at 0.02 mM and 0.05 mM, respectively. The increase induced by 0.02 mM SNAP was abolished by the addition of 0.01mM oxyhemoglobin, a scavenger of NO. [3H]Thymidine incorporation in stationary cells was also increased by SNAP. In addition, 0.02mM SNAP produced a 1.8‐3.2‐fold increase of thymidine kinase activity in exponentially growing cells.