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Failure of D‐fructose to stimulate protein biosynthesis in pancreatic islets
Author(s) -
Viñambres Concepción,
VillanuevaPeñacarrillo Maria L.,
Valverde Isabel,
Malaisse Willy J.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201601
Subject(s) - fructose , pancreatic islets , medicine , endocrinology , islet , trichloroacetic acid , stimulation , chemistry , amino acid , biosynthesis , metabolism , biochemistry , insulin , biology , enzyme
Rat pancreatic islets were incubated for 90 min with L‐[4‐3H]phenylalanine in either the absence or presence of D‐glucose and D‐fructose. Relative to basal value, D‐glucose (16.7 mM) increased the incorporation of the tritiated amino acid into trichloroacetic acid‐precipitable material. However, when D‐fructose was tested in the 80 to 240 mM concentration range, it failed to stimulate islet biosynthetic activity. Since D‐fructose causes, in the same concentration range, a dose‐related stimulation of insulin release, the dissociation between the biosynthetic and secretory responses to D‐fructose supports the view that the insulinotropic action of the ketohexose does not entail the same metabolic determinants as those operative in glucose‐stimulated islets.