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Upregulation of HLA class‐I gene transcription in K562 cells by analogs of splenopentin (SP‐5)
Author(s) -
ChatterjeeKishore Moitreyee,
Agrawal Suraksha,
Singh VK,
Mathur KB,
Agarwal Shyam S.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201541
Subject(s) - gene , downregulation and upregulation , k562 cells , transcription (linguistics) , human leukocyte antigen , microbiology and biotechnology , biology , transcription factor , electrophoretic mobility shift assay , cell culture , transcriptional regulation , chemistry , genetics , antigen , philosophy , linguistics
We report here the capacity of three analogs of Splenopentin, to up regulate transcription of the HLA ‐B7 gene in the K562 cell line. These cells normally do not transcribe the HLA class I genes. The Splenopentin analogs used in this study are effective at very low molar concentrations and are non‐toxic to cells at these levels. Electrophoretic Mobility Shift assays indicate that this transcriptional up regulation of HLA class I genes may be related to the appearance of novel class I promoter binding factors induced in the nuclei of treated cells.