Premium
Effect of cardiolipin on functional properties of isolated rat liver mitochondria
Author(s) -
Bobyleva Valentina,
Bellei Monica,
Pazienza Teresa Loredana,
Muscatello Umberto
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201491
Subject(s) - cardiolipin , mitochondrion , inner mitochondrial membrane , calcium , chemistry , microbiology and biotechnology , receptor , atpase , membrane , biochemistry , in vitro , biophysics , enzyme , biology , phospholipid , organic chemistry
The specific involvement of cardiolipin in modulating and/or controlling the activity of a number of mitochondrial carriers, enzymes and receptors is well documented; however, comparatively less understood is its role for the integrated functions of intact mitochondria. The aim of the present research was to get a better insight into this problem by investigating the effect of in vitro addition of cardiolipin on the properties of isolated liver mitochondria. The results obtained show that cardiolipin induces extensive structural and functional perturbations at the level of the inner mitochondrial membrane. In fact, addition of cardiolipin to intact mitochondria causes a significant increase of proton leak associated with a parallel increase of respiratory rate in State 4. Concomitantly, a slight uncoupling of phosphorylation associated with a moderate increase in ATPase activity is observed. Furthermore, the pore‐mediated membrane permeability to calcium is drastically modified, an effect that can be reversed by addition of cyclosporin.