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A novel property of adenine nucleotides: Sensitivity to helium‐neon laser in mitochondrial reactions
Author(s) -
Gagliardi S.,
Atlante A.,
Passarella S.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201471
Subject(s) - adenylate kinase , atp synthase , adenine nucleotide , mitochondrion , atp–adp translocase , oxidative phosphorylation , nucleotide , biochemistry , kinetics , chemistry , adenosine triphosphate , phosphorylation , biophysics , substrate level phosphorylation , inner mitochondrial membrane , enzyme , biology , physics , quantum mechanics , gene
Consideration is made here of the ability of He‐Ne laser light to affect both transport and enzymic reactions by acting on substrates. Adenine nucleotides irradiated with 3 Joules/cm2 fluence (10 mW/cm2 fluence rate) showed altered biochemical behaviour when used as substrates for certain mitochondrial reactions in isolated rat liver mitochondria: ADP/ATP antiport and ATP synthase, which allow for oxidative phosphorylation, and adenylate kinase reaction. In order to determine ATP synthase kinetics a specific method was developed which allows for calculation of ADP phosphorylation rate in intact mitochondria. While no change in ATP synthase kinetics was observed as a result of ADP irradiation, adenine nucleotides proved to be sensitive to He‐Ne laser irradiation when their interaction with ADP/ATP carrier and adenylate kinase was considered.