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Essential fatty acid metabolism in cardiomyocytes grown in media enriched with different N‐6/N‐3 fatty acid combinations
Author(s) -
Hrelia Silvana,
Biagi Pier L.,
Lorenzini Antonello,
Jimenez Josè A. Lopez,
Horrobin David F.,
Bordoni Alessandra
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201441
Subject(s) - fatty acid , polyunsaturated fatty acid , biochemistry , linoleic acid , metabolism , chemistry , fatty acid metabolism , essential fatty acid , linolenic acid , food science
We have evaluated the effects of three different 18:3n‐6, 20:5n‐3 and 22:6n‐3 fatty acid combinations on essential fatty acid (EFA) metabolism in rat cultured cardiomyocytes. The desaturating/elongating activities for linoleic (LA) and α‐linolenic acid (ALA) were evaluated by radlolabehng the cells with 1‐[14C]LA or 1‐[14C]ALA and the fatty acid pattern of cardiomyocytes was assessed by gas chromatography. LA and ALA conversion to more unsaturated metabolites was reduced by increasing respectively n‐3 and n‐6 fatty acid concentration in the media. The all three combinations used reduced the saturated and increased the polyunsaturated fatty acid content of cardiomyocytes. The n‐6/n‐3 fatty acid ratio did not change compared to control cells in cardiomyocytes receiving the highest amount of 18:3n‐6 and the lowest amounts of n‐3 fatty acids This combination may be suitable for modifying EFA desaturating/elongating activities without altering the physicochemical parameters which are related to the correct balance between n‐6 and n‐3 fatty acid content.