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Electron paramagnetic resonance studies of proteins in ozone‐treated erythrocyte membranes
Author(s) -
Wróbel Anna,
Jezierski Adam,
Gomułkiewicz Jan
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201361
Subject(s) - membrane , electron paramagnetic resonance , chemistry , ozone , spin label , covalent bond , rotational correlation time , site directed spin labeling , membrane protein , biophysics , biochemistry , analytical chemistry (journal) , nuclear magnetic resonance , chromatography , molecule , organic chemistry , biology , physics
The sulfhydryl group specific spin labels have been used to study the membrane proteins in the bovine and human erythrocyte membranes treated with ozone. The ratio hw/hs, determined from the respective peak amplitudes of the resulting EPR spectra of 3‐maleimido‐1‐oxyl‐2,2,5,5‐tetramethylpyrrolidine bound to the ozone treated membranes, was shown to be elevated following an increased ozone concentration. The ratio of the central to high‐field peak amplitude h0 / h‐1, a measure of the rotational correlation time, determined from EPR spectra of ozonized membranes covalently labeled with 4‐(iodoacetamido)‐l‐oxyl‐2,2,5,5‐tetramethylpyrrolidine was shown to be diminished. The changes in the erythrocyte membranes treated with ozone, observed for both spin labels, indicate a dose dependent increase in the mobility of membrane proteins.