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Immunoreactivities of p11 and calcyclin in baker's yeast, wheat germ and lobster
Author(s) -
Kuo W. N.,
JnBaptiste J. B.,
Zhai L.,
Chambers M. C.,
Kanadia R.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201211
Subject(s) - agarose , yeast , blot , wheat germ , microbiology and biotechnology , germ , biology , endogeny , biochemistry , chemistry , gene
After effectively eliminating the nonspecific cross‐immunoreactivity with the affinity columns of anti‐IgG agarose and IgG agarose, the potent immunoreactivities of p11 and calcyclin in wheat germ, lobster tail muscle, and three strains of baker's yeast were analyzed by Western blotting using mouse anti‐p11 and rabbit anti‐calcyclin. The occurrence of multiple bands may be due to either autolyses and/or the interactions between the p11 (or calcyclin) and other endogenous biological molecules. The results suggest not only a ubiquitous distribution and a universal Ca2+‐mediating regulatory role of p11 and calcyclin in eukaryotes, but also an evolutionary conservation of these (S‐100)‐related proteins.