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Identification of a Zn2 ‐sensitive component of Ehrlich cell plasma membrane redox system by CHAPS‐agarose‐polyacrylamide electrophoresis and in situ staining of activity
Author(s) -
RodríguezCaso Luis,
RodríguezAgudo Daniel,
CastilloOlivares Antonio del,
Márquez Javier,
de Castro Ignacio Núñez,
Medina Miguel Ángel
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201081
Subject(s) - polyacrylamide , agarose , chaps , electrophoresis , staining , membrane , chromatography , chemistry , polyacrylamide gel electrophoresis , in situ , biochemistry , biology , enzyme , polymer chemistry , organic chemistry , genetics
Abstract A procedure based on CHAPS‐agarose‐polyacrylamide electrophoresis and in situ staining of activity was used to detect a Zn2+‐sensitive component of Ehrlich cell plasma membrane redox system. The procedure is so powerful that it allows to use crude plasma membrane fractions and can be easily adapted for use in an electrophoretic approach to the purification of this protein.