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Regulation of cholesterol synthesis and esterification in primary cultures of macrophages following uptake of Chylomicron remnants
Author(s) -
Yu Kenneth C. W.,
Mamo John C. L.
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201031
Subject(s) - chylomicron , very low density lipoprotein , cholesterol , foam cell , reverse cholesterol transport , in vitro , chemistry , intermediate density lipoprotein , hmg coa reductase , biochemistry , lipoprotein , macrophage , intracellular , reductase , medicine , enzyme
The effects of chylomicron remnants (CR), β‐very‐low‐density‐lipoproteins (β‐VLDL) and low‐density‐lipoproteins (LDL) on intracellular cholesterol synthesis and esterification in primary rabbit macrophages was determined by assaying for HMG‐CoA reductase activity and cholesterol esterification. At physiological cholesterol concentrations, both CR and LDL inhibited cholesterol synthesis by almost 60% while β‐VLDL was less potent achieving only 30% inhibition. Cholesterol esterification rates were increased four‐fold by CR and LDL, whereas β‐VLDL increased esterification 14 times above controls. Qualitatively, the effect of CR on cholesterol synthesis and esterification in rabbit macrophages differs from observations in transformed macrophage cells. Quantitatively, the enhanced rates of cholesterol esterification and weak inhibition of cholesterol synthesis following β‐VLDL uptake may explain why this lipoprotein rapidly induces foam cell formation in vitro.