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Effect of polyanions on ferricytochrome c
Author(s) -
Sedlák Erik
Publication year - 1997
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549700201021
Subject(s) - chemistry , heme , fluorescence , spin states , crystallography , absorption (acoustics) , native state , photochemistry , stereochemistry , inorganic chemistry , materials science , organic chemistry , enzyme , physics , quantum mechanics , composite material
Effects of two polyanions (heparin, Nation) on absorption and CD spectra in the Soret region, and on fluorescence of ferricytochrome c were investigated. The conformational state of ferricyt c in the complexes with studied polyanions is significantly different. Whereas heparin affects the structure in the heme region of ferricyt c only slightly (ferricyt c remains in a native‐like state), the influence of Nation is more expressive (the Met‐80 ‐ heine iron bond and the deeply buried region around Trp‐59 are perturbed). Shift of the pK of the transition from neutral low‐spin state to acidic high‐spin state to higher pH values was observed.