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Intracellular distribution of polyamines in human lymphoblastoid cell line during phorbol ester‐induced differentiation
Author(s) -
Stabellini Giordano,
Creati Beatrice,
Primio Roberto Di,
Trubiani Oriana
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201941
Subject(s) - spermine , spermidine , putrescine , polyamine , biochemistry , intracellular , phorbol , cytoplasm , cell culture , cellular differentiation , tetradecanoylphorbol acetate , microbiology and biotechnology , chemistry , biology , enzyme , protein kinase c , gene , genetics
Polyamines such as putrescine, spermidine and spermine play an important role in nucleic acid metabolism. These aliphatic amines display a key role in cell‐induced transformation by carcinogenic substances. In particular, one of these, the phorbol myristate acetate, provokes cell differentiation and gives an increase of ornithindecarboxylase activity; enzyme regulating the pathways of polyamines. In this study we analyse the trend of the polyamines at cytoplasmic and nuclear level during phorbol treatment. Our results show a correlation between nuclear and cytoplasmic spermine, 3H‐Thymidine, 3H‐Leucine incorporation and cell cycle phases. These data remark that the polyamines are differently distributed into the cell during the phorbol myristate acetate‐mediated differentiation process and that the spermine is down‐regulated for to supply the increased protein biosynthesis.