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Opposite effects of basic fibroblast growth factor and heparin on heparin/heparan sulfate degrading enzymes from Flavobacterium heparinum
Author(s) -
Cavari Simone,
Vannucchi Simonetta
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201921
Subject(s) - heparin , heparan sulfate , flavobacterium , fibroblast growth factor , depolymerization , chemistry , basic fibroblast growth factor , biochemistry , substrate (aquarium) , enzyme , molar ratio , growth factor , biology , bacteria , pseudomonas , genetics , ecology , receptor , catalysis , organic chemistry
Heparinase I is normally inhibited by an excess or substrate; in this paper we describe that bFGF counteracts the inhibiting effect of the substrate and that heparinase I activity is increased in the presence of bFGF. This effect was observed using heparin concentrations ranging from 10‐9 to 10‐7 M with either a ten fold molar excess or an equimolar concentration of bFGF. In addition, the degree of depolymerization of heparan sulfate produced by heparitinase from Flavobacterium heparinum was increased in the presence of bFGF.