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Interleukin‐4 stimulates rheumatoid synovial fibroblasts to express matrix metalloproteinase‐1 (tissue collagenase) and histamine H1 receptor mRNA
Author(s) -
Zenmyo Michihisa,
Hiraoka Koji,
Sasaguri Takakazu,
Komiya Setsuro,
Inoue Akio,
Morimatsu Minoru,
Sasaguri Yasuyuki
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201903
Subject(s) - histamine , collagenase , matrix metalloproteinase , northern blot , messenger rna , interstitial collagenase , histamine h4 receptor , chemistry , metalloproteinase , receptor , matrix metalloproteinase 3 , microbiology and biotechnology , medicine , endocrinology , immunology , biology , histamine h2 receptor , enzyme , biochemistry , gene , antagonist
In the present study, we investigated the effect of interleukin‐4 (IL‐4) on metalloproteinase 1 (MMP‐1/tissue collagenase) production in human rheumatoid synovial fibroblasts. Northern blot analysis revealed that addition of IL‐4 with or without histamine stimulated the cells to increase the amount of proMMP‐1 mRNA, and the IL‐4 with histamine addition resulted in a 3.3‐fold increase compared with histamine only. Furthermore, IL‐4 itself stimulated the expression of histamine H1 receptor mRNA. These results suggest that IL‐4 may play an important role in joint destruction in RA.