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Identification and immunohistochemical localization of macrophage migration inhibitory factor in human kidney
Author(s) -
Imamura Keisaku,
Nishihira Jun,
Suzuki Masaki,
Yasuda Kazue,
Sasaki Satoshi,
Kusunoki Yukihiro,
Tochimaru Hiroyuki,
Takekoshi Yasuo
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201883
Subject(s) - macrophage migration inhibitory factor , immunohistochemistry , western blot , polyclonal antibodies , proinflammatory cytokine , kidney , biology , blot , inflammation , tumor necrosis factor alpha , microbiology and biotechnology , autocrine signalling , antibody , pathology , immunology , cytokine , medicine , cell culture , endocrinology , gene , biochemistry , genetics
Macrophage migration inhibitory factor (MIF) was the first lymphokine identified in activated T‐lymphocytes. MIF can induce proinflammatory cytokines, such as interleukin‐1 and tumor necrosis factor‐α. In this study, we identified MIF expression in a tissue specimen of a normal portion of a nephrectomized human kidney by reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blot analysis. Furthermore, immunohistochemical study using an anti‐human MIF polyclonal antibody demonstrated that MIF was mostly present in the renal tubule epithelial cells and, to a lesser extent, in Bowman's capsular epithelial cells. We also carried out immunohistochemistry on cultured human renal proximal tubule epithelial cells, which showed that MIF was present in the cytoplasm of the epithelial cells. These results suggest the possibility that MIF takes part in the mechanism of inflammation and immunological events in the human kidney.