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3‐Hydroxybutyrate decreases noradrenaline affinity for rat erythrocyte ghost β2‐adrenergic receptors
Author(s) -
Gómez G.,
Cañas X.,
FernándezLópez J. A.,
Remesar X.,
Alemany M.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201862
Subject(s) - receptor , adrenergic receptor , endocrinology , medicine , chemistry , adrenergic , membrane , catecholamine , biophysics , biology , biochemistry
The effects of physiological levels of 3‐hydroxybutyrate on noradrenaline binding to rat erythrocyte membranes were studied. The binding of noradrenaline to membranes was determined by measuring the union of 3H‐noradrenaline in the presence of varying concentrations of 3‐hydroxybutyrate. Scatchard plots of the results were used to determine that 3‐hydroxybutyrate provoked a loss of affinity of the β2‐adrenergic receptors for adrenaline. Thus high (but physiological) 3‐hydroxybutyrate levels can lower the binding of the catecholamine, thus diminishing the extent or intensity of adrenergic‐driven responses