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The methylation pattern of a cytosine DNA‐methyltransferase gene in Arabidopsis thaliana plants
Author(s) -
Kutueva Lyudmila I.,
Ashapkin Vasili V.,
Vanyushin Boris F.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201832
Subject(s) - hpaii , biology , dna methylation , methyltransferase , dna methyltransferase , microbiology and biotechnology , cytosine , methylation , arabidopsis thaliana , dna , gene , complementary dna , restriction enzyme , genetics , gene expression , mutant
Using a PCR‐amplified 5′‐end proximal 600‐bp fragment and two cDNA clones of cytosine DNA‐methyltransferase gene of Arabidopsis thaliana as specific probes in hybridization with plant DNA samples, hydrolyzed by methylation‐sensitive restriction endonucleases HpaII and MspI, it has been established that CCGG sites located in the 5′‐end proximal part of cytosine DNA‐methyltransferase gene are highly methylated at internal C and less but detectably methylated at external C residues. On the contrary, all CCGG sites in 3′‐terminal half of the coding region were found to be unmethylated at both external and internal C residues. No significant differences between methylation patterns of cytosine DNA‐methyltransferase gene in various organs (leaf, stem, flower) of the Arabidopsis thaliana plant were detected.