Premium
Expression of mRNA encoding cAMP‐specific phosphodiesterase isoforms in rat parotid glands
Author(s) -
Imai Akane,
Nashida Tomoko,
Shimomura Hiromi
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201813
Subject(s) - gene isoform , parotid gland , phosphodiesterase , alternative splicing , messenger rna , rna splicing , gene expression , microbiology and biotechnology , gene , biology , reverse transcriptase , subfamily , phosphorylation , endocrinology , medicine , chemistry , polymerase chain reaction , biochemistry , enzyme , rna , pathology
In previous reports, we have shown that cAMP‐specific phosphodiesterase (PDE4) is the major PDE in the rat parotid gland, and that PDE4 is activated by phosphorylation. In this study, we investigated the expression of PDE4 isoform genes and alternative splicing variants of PDE4D in the rat parotid gland using reverse transcriptase‐polymerase chain reaction (RT‐PCR). PDE4A, PDE4B, PDE4C and PDE4D of PDE4 subfamily were expressed. PDE4D was found to be the dominant PDE4 isoform. A weak band of PDE4C was detectable. Three alternative splicing variants (PDE4D1, PDE4D2 and PDE4D3) derived from the rat PDE4D gene were expressed in the parotid gland. These data suggested that the intracellular cAMP level is regulated by multiple response mechanisms through the activations of the PDE by phosphorylation and gene expression in the rat parotid gland.