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Isolation of toxin TsTX‐VI from Tityus serrulatus scorpion venom. Effects on the release of neurotransmitters from synaptosomes
Author(s) -
Sampaio Suely V.,
CoutinhoNetto Joaquim,
Arantes Eliane C.,
Marangoni Sergio,
Oliveira Benedito,
Giglio José R.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201811
Subject(s) - venom , toxin , scorpion , chemistry , tetrodotoxin , amino acid , scorpion toxin , neurotoxin , biochemistry , pharmacology , biology , biophysics
A detailed procedure for the purification of Tityustoxin‐VI, TxTX‐VI, from Tityus serrulatus scorpion venom is described. For comparative purposes, a second toxin, CM‐VI, obtained from the same fractionation procedure, was analyzed in parallel. Typical biochemical parameters, such as electrophoretic migration, mol. weight, amino acid composition and N‐terminal sequence (first 42 amino acid residues out of a total of approx. 60) were determined for both. Our data showed that CM‐VI is identical or extremely homologous to γ‐toxin (TsTX‐I), the highly toxic major toxin from T. serrulatus venom. TsTX‐VI was less toxic, although still effective at inducing an allergic reaction, lacrymation and contraction of the hind legs of mice. Both toxins produced a dose dependent release of the neurotransmitters glutamic acid and gamma aminobutyric acid from rat brain synaptosomes, this effect being blocked by tetrodotoxin.