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A phylogenetic study of Rubrobacter radiotolerans by sequence analysis of the 16S ribosomal RNA gene
Author(s) -
Kausar Javed,
Ohyama Yoshihiko,
Yamamoto Osamu
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201771
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , arthrobacter , ribosomal rna , gene , genetics , sequence analysis , phylogenetic relationship , bacteria , microbiology and biotechnology
Partial sequence of the 16S ribosomal RNA gene of an extremely high radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotoleransby chemical characteristics) was determined by PCR‐amplification from a small amount of heat‐lysed biomass, followed by direct sequencing of the PCR product. The sequence was aligned with seven species of Arthrobacter and also with representatives of various other bacterial groups. R. radiotolerans was confirmed to be out of the Arthrobacter group justifying the reclassification. Moreover, it has an individual position among the selected representatives being closer to the eubacterial groups.