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Subcellular redistribution of hsp72 protein during cisplatin‐induced apoptosis in HeLa cells
Author(s) -
MeléndezZajgla Jorge,
García Claudia,
Maldonado Vilma
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201742
Subject(s) - hela , apoptosis , cisplatin , heat shock protein , redistribution (election) , microbiology and biotechnology , reactive oxygen species , programmed cell death , immunofluorescence , chemistry , dna damage , cell , biology , dna , antibody , biochemistry , immunology , genetics , chemotherapy , politics , gene , political science , law
Exposure of HeLa cells to cisplatin results in the activation of apoptotic cell death. This drug induces DNA damage and generates reactive oxygen intermediates. Since cisplatin is highly reactive and binds to diverse proteins, it could create abnormal protein structures or nonspecific aggregates. For these reasons, we analyzed the expression and subcelullar distribution of hsp72, a heat‐shock protein that enables cells under stress to cope with damaged proteins. We did not observe any changes in the expression of hsp72 protein, although, by immunofluorescence studies, we detected a dramatic redistribution of the protein. These results and its probable relevance in the drug‐induced apoptotic phenomenon are discussed.