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Susceptibility of growth factors to degradation by Helicobacter pylori protease: Effect of ebrotidine and sucralfate
Author(s) -
Slomiany B. L.,
Piotrowski J.,
Slomiany A.
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201702
Subject(s) - sucralfate , protease , helicobacter pylori , chemistry , platelet derived growth factor receptor , microbiology and biotechnology , growth factor , biology , biochemistry , enzyme , medicine , receptor
In this study, we investigated the susceptibility of the growth factors (EGF, bFGF, TGFß and PDGF) to degradation by the protease elaborated by H. pylori and assessed the effect of antiulcer agents, ebrotidine and sucralfate, on this enzymatic action of the bacterium. The colonies of H. pylori were washed with saline, filtered to retain the bacteria, and the filtrate used as an enzyme source. The assays revealed that while EGF and ßFGF showed only marginal (5‐7%) susceptibility to H. pylori protease, a 61.7% degradation occurred with PDGF and 62.3% with TGFß. Introduction of ebrotidine or sucralfate to the assay system led to the reduction in the rate of growth factors degradation, which at 50µg/ml ebrotidine reached the value of 85.1% for PDGF and 88.6% for TGFß, while with sucralfate the optimal inhibition of PDGF (79.6%) and TGFß (82.7%) degradation occurred at 200µg/ml. The results demonstrate that PDGF and TGFß are susceptible to H. pylori degradation and that antiulcer agents, ebrotidine and sucralfate are capable of counteracting this effect.