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Molecular cloning and expression of a cDNA encoding a protein tyrosine phosphatase from mouse brain
Author(s) -
Park Kyung Soo,
Kim Yeon Woong,
Kim Choong Won,
Kang Dae Ook,
Byun Si Myung
Publication year - 1996
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549600201663
Subject(s) - complementary dna , microbiology and biotechnology , cdna library , open reading frame , biology , northern blot , protein tyrosine phosphatase , molecular cloning , cloning (programming) , nucleic acid sequence , peptide sequence , tyrosine , amino acid , southern blot , biochemistry , gene , computer science , programming language
A mouse brain cDNA library was screened with a radioisotope‐labeled probe of human placental protein tyrosine phosphatase. The isolated clone (MBPTP1B) was found to contain an open reading frame of 1,296 nucleotides as well as 5′ (709 nucleotides) and 3′ (341 nucleotides) non‐coding regions. This cDNA encodes a PTP of 432 amino acids having a mass of 49,563 daltons and exhibiting 83% and 93.5% sequence identity to that of human PTP1B and rat PTP1, respectively. The expression of the cDNA in yeast was identified by western blot analysis and PTP activity assay.